| 1 | |  Union Omaha SC | 28 | 14 | 9 | 5 | 44 | 22 | 22 | 51 | G02:00 | 17/10/21 | R 1  Union Omaha SC 4 North Carolina FC 1 M03:00 | 21/10/21 | R 1 Union Omaha SC 1 Forward Madison FC 2 G02:00 | 24/10/21 | R 1 Union Omaha SC 2 Richmond Kickers 0 B03:30 | 31/10/21 | R 1 North Texas SC 1 Union Omaha SC 1 G01:00 | 14/11/21 | R semifinal Union Omaha SC 6 FC Tucson 1 G01:00 | 21/11/21 | R final Union Omaha SC 3 Greenville Triumph SC 0 |
| 2 | |  Greenville Triumph SC | 28 | 12 | 9 | 7 | 36 | 29 | 7 | 45 | G02:00 | 10/10/21 | R 1 Union Omaha SC 0 Greenville Triumph SC 1 G02:00 | 17/10/21 | R 1 Greenville Triumph SC 1 New England Revolution II 0 B02:00 | 24/10/21 | R 1 Greenville Triumph SC 0 Chattanooga Red Wolves SC 0 G02:00 | 31/10/21 | R 1 North Carolina FC 0 Greenville Triumph SC 2 G03:00 | 14/11/21 | R semifinal Greenville Triumph SC 2 Chattanooga Red Wolves SC 0 M01:00 | 21/11/21 | R final Union Omaha SC 3 Greenville Triumph SC 0 |
| 3 | |  Chattanooga Red Wolves SC | 28 | 11 | 11 | 6 | 37 | 29 | 8 | 44 | G22:00 | 09/10/21 | R 1 North Carolina FC 2 Chattanooga Red Wolves SC 3 M02:00 | 17/10/21 | R 1 Chattanooga Red Wolves SC 1 Toronto FC II 2 B02:00 | 24/10/21 | R 1 Greenville Triumph SC 0 Chattanooga Red Wolves SC 0 M03:00 | 31/10/21 | R 1 Forward Madison FC 2 Chattanooga Red Wolves SC 1 G02:00 | 07/11/21 | R quarterfinal Chattanooga Red Wolves SC 2 North Texas SC 1 M03:00 | 14/11/21 | R semifinal Greenville Triumph SC 2 Chattanooga Red Wolves SC 0 |
| 4 | |  FC Tucson | 28 | 11 | 7 | 10 | 44 | 42 | 2 | 40 | M05:00 | 14/10/21 | R 1 FC Tucson 0 North Carolina FC 2 G05:00 | 17/10/21 | R 1 FC Tucson 3 South Georgia Tormenta FC 1 B03:00 | 24/10/21 | R 1 Forward Madison FC 2 FC Tucson 2 G05:00 | 31/10/21 | R 1 FC Tucson 4 Richmond Kickers 2 G05:00 | 07/11/21 | R quarterfinal FC Tucson 1 Richmond Kickers 0 M01:00 | 14/11/21 | R semifinal Union Omaha SC 6 FC Tucson 1 |
| 5 | |  Richmond Kickers | 28 | 11 | 7 | 10 | 35 | 36 | -1 | 40 | G01:30 | 07/10/21 | R 1 Richmond Kickers 1 Forward Madison FC 0 G02:00 | 11/10/21 | R 1 North Texas SC 1 Richmond Kickers 2 G01:30 | 17/10/21 | R 1 Richmond Kickers 3 Forward Madison FC 2 M02:00 | 24/10/21 | R 1 Union Omaha SC 2 Richmond Kickers 0 M05:00 | 31/10/21 | R 1 FC Tucson 4 Richmond Kickers 2 M05:00 | 07/11/21 | R quarterfinal FC Tucson 1 Richmond Kickers 0 |
| 6 | |  North Texas SC | 28 | 10 | 10 | 8 | 40 | 32 | 8 | 40 | G05:00 | 03/10/21 | R 1 FC Tucson 0 North Texas SC 2 M02:00 | 11/10/21 | R 1 North Texas SC 1 Richmond Kickers 2 G02:30 | 18/10/21 | R 1 Fort Lauderdale CF 0 North Texas SC 1 G03:30 | 24/10/21 | R 1 North Texas SC 4 New England Revolution II 1 B03:30 | 31/10/21 | R 1 North Texas SC 1 Union Omaha SC 1 M02:00 | 07/11/21 | R quarterfinal Chattanooga Red Wolves SC 2 North Texas SC 1 |
| 7 | |  Toronto FC II | 28 | 10 | 8 | 10 | 34 | 32 | 2 | 38 | M01:30 | 26/09/21 | R 1 Richmond Kickers 1 Toronto FC II 0 G23:00 | 01/10/21 | R 1 Toronto FC II 3 North Carolina FC 0 G23:00 | 08/10/21 | R 1 Toronto FC II 1 New England Revolution II 0 G02:00 | 17/10/21 | R 1 Chattanooga Red Wolves SC 1 Toronto FC II 2 M23:00 | 22/10/21 | R 1 Toronto FC II 1 Fort Lauderdale CF 2 G22:00 | 29/10/21 | R 1 Toronto FC II 1 New England Revolution II 0 |
| 8 | |  New England Revolution II | 28 | 11 | 4 | 13 | 33 | 39 | -6 | 37 | G00:00 | 02/10/21 | R 1 New England Revolution II 1 Forward Madison FC 0 M23:00 | 08/10/21 | R 1 Toronto FC II 1 New England Revolution II 0 G00:00 | 13/10/21 | R 1 New England Revolution II 1 Forward Madison FC 0 M02:00 | 17/10/21 | R 1 Greenville Triumph SC 1 New England Revolution II 0 M03:30 | 24/10/21 | R 1 North Texas SC 4 New England Revolution II 1 M22:00 | 29/10/21 | R 1 Toronto FC II 1 New England Revolution II 0 |
| 9 | |  Forward Madison FC | 28 | 8 | 12 | 8 | 32 | 34 | -2 | 36 | M01:30 | 07/10/21 | R 1 Richmond Kickers 1 Forward Madison FC 0 M00:00 | 13/10/21 | R 1 New England Revolution II 1 Forward Madison FC 0 M01:30 | 17/10/21 | R 1 Richmond Kickers 3 Forward Madison FC 2 G03:00 | 21/10/21 | R 1 Union Omaha SC 1 Forward Madison FC 2 B03:00 | 24/10/21 | R 1 Forward Madison FC 2 FC Tucson 2 G03:00 | 31/10/21 | R 1 Forward Madison FC 2 Chattanooga Red Wolves SC 1 |
| 10 | |  Fort Lauderdale CF | 28 | 8 | 7 | 13 | 40 | 49 | -9 | 31 | B02:30 | 20/09/21 | R 1 Fort Lauderdale CF 2 South Georgia Tormenta FC 2 M02:00 | 02/10/21 | R 1 Greenville Triumph SC 1 Fort Lauderdale CF 0 M03:00 | 07/10/21 | R 1 Union Omaha SC 4 Fort Lauderdale CF 1 B02:30 | 11/10/21 | R 1 Fort Lauderdale CF 4 South Georgia Tormenta FC 4 M02:30 | 18/10/21 | R 1 Fort Lauderdale CF 0 North Texas SC 1 G23:00 | 22/10/21 | R 1 Toronto FC II 1 Fort Lauderdale CF 2 |
| 11 | |  South Georgia Tormenta FC | 28 | 8 | 6 | 14 | 36 | 47 | -11 | 30 | G03:00 | 12/09/21 | R 1 South Georgia Tormenta FC 2 North Texas SC 0 B02:30 | 20/09/21 | R 1 Fort Lauderdale CF 2 South Georgia Tormenta FC 2 M03:00 | 02/10/21 | R 1 South Georgia Tormenta FC 0 Union Omaha SC 4 B02:30 | 11/10/21 | R 1 Fort Lauderdale CF 4 South Georgia Tormenta FC 4 M05:00 | 17/10/21 | R 1 FC Tucson 3 South Georgia Tormenta FC 1 M02:00 | 24/10/21 | R 1 North Carolina FC 1 South Georgia Tormenta FC 0 |
| 12 | |  North Carolina FC | 28 | 7 | 4 | 17 | 30 | 50 | -20 | 25 | M23:00 | 01/10/21 | R 1 Toronto FC II 3 North Carolina FC 0 M22:00 | 09/10/21 | R 1 North Carolina FC 2 Chattanooga Red Wolves SC 3 G05:00 | 14/10/21 | R 1 FC Tucson 0 North Carolina FC 2 M02:00 | 17/10/21 | R 1 Union Omaha SC 4 North Carolina FC 1 G02:00 | 24/10/21 | R 1 North Carolina FC 1 South Georgia Tormenta FC 0 M02:00 | 31/10/21 | R 1 North Carolina FC 0 Greenville Triumph SC 2 |
